Comprehensive charac¬terization of cultivated in vitro Deschampsia antarctica E. Desv. plants with different chromosome numbers

Abstract

Abstract — D. antarctica E. Desv. plants cultivated in vitro were analyzed for the chromosome number, leaf length, and efficiency of callus formation. Most of the studied genotypes had a typical diploid chromosome number. However, a hypotriploid and a plant with B chromosomes demonstrated mixoploidy caused by the presence of a significant proportion (up to 15–25%) of aneuploid cells. Jaccard genetic distances between the plants determined from the data of ISSR- and IRAP-PCR analyses were within the range of 0.0323 to 0.1803. Furthermore, genetic distances between the specimens with atypical karyotype and diploids did not exceed the paired distances within the group of diploid plants. Variations in the leaf length and growth parameters of the plants were characterized. Plants with different chromosome numbers differed in the leaf length and efficiency of callus formation. Obtained results may indicate relationships between the chromosome number, studied morphometric parameters, and efficiency of callus formation in the analyzed D. antarctica plants.