Вплив ксенобіотиків на активність ферментів антиоксидантної системи в тканинах коропа

Abstract

Наведені дані про активність антиоксидантних ферментів - супероксиддисмутази (СОД), глутатіонпероксидази (ГП) і каталази у печінці та білих м’язах коропа за дії зенкору та натрій лаурилсульфату.

Приведены данные об активности антиоксидантных ферментов - супероксиддисмутазы, глутатионпероксидазы и каталазы в печени и белых мышцах карпа при разных условиях содержания.

The article provides the data obtained in the study of antioxidant enzymatic activities - superoxide dismutase, glutathione peroxidase and catalase in the liver and white muscle of carp under different conditions. The aim of the research is to analyze the influence of toxic conditions of maintenance the activity of antioxidant enzymes of white muscles and a liver of a carp (Cyprinus carpio L.). Xenobiotics is commonly used in agriculture and everyday life. The subject of the research is the carp (Cyprinus carpio L.). One of the representatives of surfactants is sodium lauryl sulfate. It is a mixture of alkyl sodium sulfate and contains 55-85% of sodium dodecyl sulfate. The main feature of the mechanism of sensibilizing action of preparation on the basis of surfactants is that the detergents interact with a cell membrane of the organs and the muscles and this process is accompanied by the replacement of enzymatic activity and sudden increase in permeability of cell membrane. Herbicide zenkor (metribuzin, 4-amino-3-metylmercapto-6 tert-butyl-1,2,4-triazine-5) is known as an efficient potato pest control agent. Intoxication of zenkor affects the behavior, causes histological and energetical changes of the fish organism. Adding zenkor and surfactants containing sodium lauryl sulfate into the water of the aquarium triggers changes of activity of all three enzymes under research - superoxide dismutase, catalase and glutathione peroxidase in the muscles of the capr. The peak of enzymatic activities under toxical influence of zenkor is observed on the example of superoxide dismutase of the liver (up to 46.15% in comparison with the control). The enzymes of white muscles show less favourable effect of the herbicide (increase of the activity of the glutathione peroxidase to 34.65% in comparison with the control). As a result of the research study, sodium lauryl sulfate synthetical detergent causes the greatest changes of enzymatic activities of the liver superoxide dismutase (the activity increasing up to 51.38% in comparison with the control group of fish). The enzymes of the white muscles of the carp proved to be more sensitive to the influence of zenkor (34.65%). The multistage antioxidant system securing the cell protection relies on complex biochemical processes and thus enables the optimal metabolic balance of the cell.